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Image Search Results
Journal: Hippocampus
Article Title: Septohippocampal pathways contribute to system consolidation of a spatial memory: sequential implication of GABAergic and cholinergic neurons.
doi: 10.1002/hipo.20837
Figure Lengend Snippet: FIGURE 4. Representative images of ChAT (top) and Parv (bottom) immunostainings in SHAM (A and E), SAP (B and F), OREX (C and G), and SAP/OREX (D and G) rats. For anti-Parv immunostaining, a slice showing nonspecific staining (no primary antibody used during incubation) is also shown. In the MS/vDBB regions (plate modified from Paxinos and Watson, 1998), the two squares on each side delimit the regions where ODs for anti-Parv immunostaning were measured. Cholinergic neurons were mark- edly depleted in SAP (C) and SAP/OREX (D) and relatively spared in OREX (B) rats. GABA neurons were markedly depleted in OREX (G) and SAP/OREX (H) and relatively preserved in SAP (F) rats. Both neuronal populations were unaltered in SHAM animals (A and E).
Article Snippet: As primary antibodies, we used a goat polyclonal antibody directed against ChAT (1:500; Chemicon International, Temecula, CA) and a mouse monoclonal antibody directed against Parv (1:4000; Sigma-Aldrich, P 3088, St. Louis, MO), whereas donkey anti-goat (ChAT) and
Techniques: Immunostaining, Staining, Incubation